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Service

​​The coupling of liquid chromatography to tandem mass spectrometry enables rapid, specific and highly sensitive analysis of biologically active metabolites, peptides and proteins in bodily fluids.

We offer a wide range of methods, including specialized preanalytic assays, to support precise and reliable proteomic and metabolomic analyses. Methods developed in the clinical mass spectrometry section were validated for the analysis in bodily fluids, tissues and cells.

If you are interested one of our established approaches, please fill out the study form​ (internal and external document) and send it to lecms@medizin.uni-leipzig.de​.

         

Metabolomics

​Contact:
Dr. Alexander Gaudl
E-Mail: Alexander.Gaudl@medizin.uni-leipzig.de​
Phone: +49 341​ - 97 22495

​MxP500 Quant kit by Biocrates

We offer the MxP500 Quant kit by Biocrates for targeted analysis of a metabolite panel covering more than 1000 metabolites from various biochemical classes. The commercial test kit offers the largest combination of lipids and small molecules for quantitative metabolomics profiling in a single kit. 

​Targeted Metabolomics

The following table presents the available mass spectrometric applications for specific (groups of) metabolites:

Analyte classPathwaySample volume
Sterolscholesterol synthesis10 ul serum
​cholesterol resorption4.5 ul dried whole blood, tissue, plaque
Steroid hormonesSteroid metabolism100 ul serum, urin, sputum
Oxysterolsoxidative stress, cholesterol metabolism80 ul plasma, tissue
Shingolipidssphingolipid metabolism, cell membrane signalling50 ul plasma, tissue
Endocannabinoids 50 ul plasma
Amino acids (AS) and acylcarnitineAS metabolism, ketogenic AS3 ul dried whole blood
​carnitine shuttle10 ul serum or plasma
​fatty acid oxidation10 ul urine
Long-chain fatty acids, eicosanoids and resolvinsCOX, LOX, EOX, ROS200 ul plasma, tissue
TMAO, cholin, betainGut derived metabolites80 ul plasma
ApolipoproteinsLipoprotein metabolism, LDL, VDL, HDL3 ul serum​


Proteomics

​​Contact:
Lydia Kollhoff
E-Mail: Lydia.Kollhoff@medizin.uni-leipzig.de​
Phone: +49 341​ - 97 22521

Targeted proteomics

​​Our highly standardized LC-MS/MS method for apolipoprotein profiling enables simultaneous quantification of 12 apolipoproteins with optimized sample preparation time and high precision and can be applied to a variety of clinical samples.

Untargeted Proteomics

​

We use data-dependent acquisition (DDA) and data-independent acquisition approaches (DIA or SWATH) for identification of proteins from tissues and cell experiments.

We also established a robust and reproducible high-resolution LC-MS/MS platform to analyse metabolomics research questions in an untargeted manner. Here we employ the use of DIA methods to analyse metabolites from cell culture or tissue samples with the aim of hypothesis generation (doi: 10.1016/j.csbj.2025.05.046)​.

Routine diagnostics

The Section Clinical Mass Spectrometry is closely working together with the Institute of Laboratory Medicine Leipzig and Clinical Diagnostic as well as the Saxony Screening Centre in Leipzig, in charge for routine diagnostics and new-born screening. For further information on these topics please visit:​

https://www.uniklinikum-leipzig.de/einrichtungen/labormedizin/leistungsspektrum

Liebigstr. 27a, Sockelgeschoss
04103 Leipzig
Phone:
+49 341 97 22254
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