Institute of Anatomy

Human Test-Systems

Mouse models and primary cell culture are common models in the field of tumor research and build the foundation for clinical therapy. The results gained by those methods are however poorly adaptable to the human organism due to inter-species differences and intercellular mechanisms. Further, the high impact of heterogeneity, immunology and stroma associated modulations in tumor biology require new strategies to investigate complex tissue equilibration. Organotypic slice cultures of human cancers are suitable to obtain an enhanced understanding of certain inter-cellular processes and thus can reduce the translational steps from cellular and mouse-based research towards clinical implementation.

The focus of this BMBF-funded project relies on the investigation of tumor-related mechanisms of resistance and to build up a patient-oriented tool to predict susceptibility for certain therapies.

	Prof. Dr. Sonja Kallendrusch Publications E-Mail: sonja.kallendrusch@medizin.uni-leipzig.de
	Prof. Dr. Ingo Bechmann Publications E-Mail: ingo.bechmann@medizin.uni-leipzig.de

Team

Susann Hähnel (former PhD student)
Rasmus Sönnichsen (former MD student)
Laura Henning, MD student
Sebastian Prill (former MD student)
Jakob Rebstock, MD student
Franziska Köhler, MD student
Anja Tennemann (former TA)

Partners

Dr. Felicitas Merz

Prof. F. Gaunitz
Prof. J. Meixensberger
Prof. I. Gockel
Prof. U. Eichfeld
Prof. A. Dietz
Prof. G. Behre
Prof. Ch. Wittekind
S. Krämer
A. Monecke
Ch. Eisenlöffel

Prof. F. Lordick
PD U. Hacker
J. Körfer

Prof. A. Weimann
C. Kubiek

Dr. C. Stäubert

Prof. H. Brüning

Selected publications

Sönnichsen R, Henning L, Blaschke V, Winter K, Körfer J, Hähnel S, Monecke A, Wittekind C, Jansen-Winkeln B, Thieme R, Gockel I, Grosser K, Weimann A, Kubick C, Wiechmann V, Aigner A, Bechmann I, Lordick F, Kallendrusch S (2017) Individual susceptibility analysis using patient-derived tissue slices of colorectal carcinoma. Clinical Colorectal cancer Nov;11 in Press. doi: 10.1016/j.clcc.2017.11.002

Kallendrusch S, Körfer J, Lordick F, Bechmann I (2017) Human tumor slice cultures for cancer research and drug testing. Integr Cancer Sci Therap 4. doi: 10.15761/ICST.1000251

Kallendrusch S, Merz F, Mayr S, Bechmann I, Zink M (2017) Long-term Tissue Culture of Adult Brain and Spleen Slices on Nanostructured Scaffolds. Advanced Healthcare Materials May;6(9). doi: 10.1002/adhm.201601336

Körfer J, Kallendrusch S, Merz F, Wittekind C, Kubick C, Kassahun WT, Schumacher G, Moebius C, Gaßler N, Schopow N, Geister D, Wiechmann V, Weimann A, Eckmann C, Aigner A, Bechmann I, Lordick F (2016) Organotypic slice cultures of human gastric and esophagogastric junction cancer. Cancer Med Jul;5(7):1444-53. doi: 10.1002/cam4.720

Kallendrusch S, Schopow N, Stadler SC, Büning H, Hacker UT (2016) Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures. Kallendrusch S, Schopow N, Stadler SC, Büning H, Hacker UT (2016) Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures. Hum Gene Ther Methods 27(5):171-173. doi: 10.1089/hgtb.2016.137

Merz F, Gaunitz F, Dehghani F, Renner C, Meixensberger J, Gutenberg A, Giese A, Schopow K, Hellwig C, Schäfer M, Bauer M, Stöcker H, Taucher-Scholz G, Durante M, Bechmann I (2013) Organotypic slice cultures of human glioblastoma reveal different susceptibility to treatments. Neuro-Oncology Jun;15(6):670-81. doi: 10.1093/neuonc/not003

Gerlach MM, Merz F, Wichmann G, Kubick C, Wittekind C, Lordick F, Dietz A, Bechmann I (2014) Slice cultures from head and neck squamous cell carcinoma: a novel test system for drug susceptibility and mechanisms of resistance. Br J Cancer Jan 21;110(2):479-88. doi: 10.1038/bjc.2013.700.

Nitsch R∗, Bechmann I∗, Deisz RA, Haas D, Lehmann TN, Wendling U, Zipp F (2000) Human brain-cell death induced by tumour-necrosis-factor-related apoptosis-inducing ligand (TRAIL). Lancet Sep 2; 356(9232):827-8. doi: 10.1016/S0140-6736(00)02659-3