Human Test-Systems

• ​Susann Hähnel (ehemalige Doktorandin​)
• Rasmus Sönnichsen (ehemaliger Medizinstudent)
• Laura Hennig, Medizinstudentin
• Sebastian Prill (ehemaliger Doktorand)
• Jakob Rebstock, Medizinstudent
• Franziska Köhler, Medizinstudentin
• Anja Tennemann (ehemalige TA​)​

Mouse models and primary cell culture are common models in the field of tumor research and build the foundation for clinical therapy. The results gained by those methods are however poorly adaptable to the human organism due to inter-species differences and intercellular mechanisms. Further, the high impact of heterogeneity, immunology and stroma associated modulations in tumor biology require new strategies to investigate complex tissue equilibration. Organotypic slice cultures of human cancers are suitable to obtain an enhanced understanding of certain inter-cellular processes and thus can reduce the translational steps from cellular and mouse-based research towards clinical implementation.

The focus of this BMBF-funded project relies on the investigation of tumor-related mechanisms of resistance and to build up a patient-oriented tool to predict susceptibility for certain therapies.​

Sönnichsen R, Henning L, Blaschke V, Winter K, Körfer J, Hähnel S, Monecke A, Wittekind C, Jansen-Winkeln B, Thieme R, Gockel I, Grosser K, Weimann A, Kubick C, Wiechmann V, Aigner A, Bechmann I, Lordick F, Kallendrusch S (2017) Individual susceptibility analysis using patient-derived tissue slices of colorectal carcinoma. Clinical Colorectal cancer Nov;11 in Press. doi: 10.1016/j.clcc.2017.11.002

Kallendrusch S, Körfer J, Lordick F, Bechmann I (2017) Human tumor slice cultures for cancer research and drug testing. Integr Cancer Sci Therap 4. doi: 10.15761/ICST.1000251​

Kallendrusch S, Merz F, Mayr S, Bechmann I, Zink M (2​017) Long-term Tissue Culture of Adult Brain and Spleen Slices on Nanostructured Scaffolds. Advanced Healthcare Materials May;6(9). doi: 10.1002/adhm.201601336

Körfer J, Kallendrusch S, Merz F, Wittekind C, Kubick C, Kassahun WT, Schumacher G, Moebius C, Gaßler N, Schopow N, Geister D, Wiechmann V, Weimann A, Eckmann C, Aigner A, Bechmann I, Lordick F (2016) Organotypic slice cultures of human gastric and esophagogastric junction cancer. Cancer Med Jul;5(7):1444-53. doi: 10.1002/cam4.720

Kallendrusch S, Schopow N, Stadler SC, Büning H​, Hacker UT (2016) Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures. Kallendrusch ​S, Schopow N, Stadler SC, Büning H, Hacker UT (2016) Adeno-Associated Viral Vectors Transduce Mature Human Adipocytes in Three-Dimensional Slice Cultures. Hum Gene Ther Methods 27(5):171-173. doi: 10.1089/hgtb.2016.137

Merz F, Gaunitz F, Dehghani F, Renner C, Meixensberger J, Gutenberg A, Giese A, Schopow K, Hellwig C, Schäfer M, Bauer M, Stöcker H, Taucher-Scholz G, Durante M, Bechmann I (2013) Organotypic slice cultures of human glioblastoma reveal different susceptibility to treatments. Neuro-Oncology Jun;15(6):670-81. doi: 10.1093/neuonc/not003

Gerlach MM, Merz F, Wichmann G, Kubick C, Wittekind C, Lordick F, Dietz A, Bechmann I (2014) Slice cultures from head and neck squamous cell carcinoma: a novel test system for drug susceptibility and mechanisms of resistance. Br J Cancer Jan 21;110(2):479-88. doi: 10.1038/bjc.2013.700.​

Nitsch R∗, Bechmann I∗, Deisz RA, Haas D, Lehmann TN, Wendling U, Zipp F (2000) Human brain-cell death induced by tumour-necrosis-factor-related apoptosis-inducing ligand (TRAIL). ​Lancet Sep 2; 356(9232):827-8. doi: 10.1016/S0140-6736(00)02659-3​​​​​